{"id":9763,"date":"2026-04-08T12:04:53","date_gmt":"2026-04-08T12:04:53","guid":{"rendered":"https:\/\/www.proefschriftmaken.nl\/portfolio\/nisreen-okba\/"},"modified":"2026-04-23T07:57:12","modified_gmt":"2026-04-23T07:57:12","slug":"nisreen-okba","status":"publish","type":"us_portfolio","link":"https:\/\/www.proefschriftmaken.nl\/en\/portfolio\/nisreen-okba\/","title":{"rendered":"Nisreen Okba"},"content":{"rendered":"","protected":false},"excerpt":{"rendered":"","protected":false},"author":8,"featured_media":13100,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"us_portfolio_category":[45],"class_list":["post-9763","us_portfolio","type-us_portfolio","status-publish","has-post-thumbnail","hentry","us_portfolio_category-new-template"],"acf":{"naam_van_het_proefschift":"Mapping MERS-CoV Immune Responses","samenvatting":"Tot voor kort was de respons op nieuwe opkomende infecties over het algemeen reactief, waardoor de tijdige beschikbaarheid van interventiemaatregelen werd belemmerd met als gevolg dat het veel moeite kan kosten uitbraken onder controle te krijgen. Vanwege de tijd die nodig is om een kandidaat-vaccin te ontwikkelen, te testen en in licentie te nemen (enkele jaren) missen we meestal de piek van een uitbraak die vaak slechts weken tot maanden kan duren. Door het ontwikkelen van platformtechnologie\u00ebn, die kunnen worden ingezet bij uitbraken, kunnen antilichamen en vaccins mogelijk sneller gegenereerd worden. Dit zal de tijdige levering van therapeutische en preventieve producten voor opkomende virussen potentieel mogelijk maken, waardoor de verspreiding van pathogenen wordt beperkt en de menselijke en economische tol wordt verminderd. In dit proefschrift werd de ontwikkeling van platforms voor de snelle generatie van tegenmaatregelen voor een opkomend coronavirus, het Middle East respiratoir syndroom coronavirus (MERS-CoV), behandeld als onderdeel van een zo\u00f6notische paraatheidsbenadering. De aanpak die is ge\u00efmplementeerd voor het ontwerpen van vaccins was een immuun-gecorreleerde benadering, waarbij we testen ontwikkelden en valideren om belangrijke virale immunogene antigenen te identificeren (hoofdstuk 2). Dit werd gevolgd door verdere karakterisering van deze immunogene domeinen met behulp van specifieke antilichamen, nanobodies (hoofdstuk 3). Daarna werden kandidaat-vaccins rationeel ontworpen en getest op hun beschermende werkzaamheid in diermodellen (hoofdstuk 4). Dit hele proces omvatte de ontwikkeling van platforms voor snelle generatie van serologische assays, nanobodies en zware keten antilichamen, evenals subunit vaccins. Deze platforms kunnen de basis vormen voor de snelle ontwikkeling van diagnostiek, therapeutica en vaccins voor toekomstige opkomende virussen.","summary":"Our approach to emerging infections has generally been a reactive post-emergence response, hampering the availability of intervention measures in due time to have substantial impact on outbreaks. Due to the time it takes to develop, test and license a vaccine candidate which can take years, we mostly miss the peak of an outbreak which can last for weeks to months. The presence of licensed platforms that can be deployed in outbreaks for the rapid generation of therapeutic antibodies and vaccines can reduce the time to develop these countermeasures. This will allow the timely supply of therapeutic and preventive products for emerging viruses, thereby limiting their spread and reducing the human and economic toll. In this thesis, the development of platforms for the rapid generation of countermeasures for an emerging zoonotic coronavirus, Middle East respiratory syndrome coronavirus (MERS-CoV), was addressed as part of a zoonotic preparedness approach. The approach implemented for vaccine design was an immune-correlate-guided approach, whereby we developed and validated assays to identify key viral immunogenic subunits (Chapter 2). This was followed by further characterization of these immunogenic domains using specific single domain antibodies (VHHs) (Chapter 3). Following that, vaccine candidates were rationally designed and tested for their protective efficacy in animal models (Chapter 4). This process involved the development of platforms for rapid generation of serological assays, VHHs and HCAbs, as well as subunit protein immunogens. These platforms can be the basis for the rapid development of diagnostics, therapeutics and vaccines for future emerging viruses.","auteur":"Nisreen Okba","auteur_slug":"nisreen-okba","publicatiedatum":"28 mei 2020","taal":"EN","url_flipbook":"https:\/\/ebook.proefschriftmaken.nl\/ebook\/nisreenokba?iframe=true","url_download_pdf":"","url_epub":"","ordernummer":"FTP-202604081201","isbn":"978-94-6380-826-2","doi_nummer":"","naam_universiteit":"Erasmus Universiteit Rotterdam","afbeeldingen":13100,"naam_student:":"","binnenwerk":"","universiteit":"Erasmus Universiteit Rotterdam","cover":"","afwerking":"","cover_afwerking":"","design":""},"_links":{"self":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio\/9763","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio"}],"about":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/types\/us_portfolio"}],"author":[{"embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/comments?post=9763"}],"version-history":[{"count":1,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio\/9763\/revisions"}],"predecessor-version":[{"id":9766,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio\/9763\/revisions\/9766"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/media\/13100"}],"wp:attachment":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/media?parent=9763"}],"wp:term":[{"taxonomy":"us_portfolio_category","embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio_category?post=9763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}