{"id":8611,"date":"2026-04-06T09:25:52","date_gmt":"2026-04-06T09:25:52","guid":{"rendered":"https:\/\/www.proefschriftmaken.nl\/portfolio\/linde-kampers\/"},"modified":"2026-04-23T08:43:06","modified_gmt":"2026-04-23T08:43:06","slug":"linde-kampers","status":"publish","type":"us_portfolio","link":"https:\/\/www.proefschriftmaken.nl\/en\/portfolio\/linde-kampers\/","title":{"rendered":"Linde Kampers"},"content":{"rendered":"","protected":false},"excerpt":{"rendered":"","protected":false},"author":8,"featured_media":13815,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"us_portfolio_category":[45],"class_list":["post-8611","us_portfolio","type-us_portfolio","status-publish","has-post-thumbnail","hentry","us_portfolio_category-new-template"],"acf":{"naam_van_het_proefschift":"Microbial Lifestyle Engineering","samenvatting":"Door de drastische gevolgen die klimaatverandering teweeg kan brengen is duurzaamheid wereldwijd al jaren van hoge prioriteit. Microben hebben een groot aandeel in de klimaatbalans omdat ze toxische verontreinigingen uit de natuur kunnen filteren. Microben worden in industri\u00eble processen toegepast om de industrie te verduurzamen. Niet elk micro-organisme is aantrekkelijk voor industri\u00eble applicatie. Het veld van Systeembiologie in Biotechnologie biedt nieuwe methoden om hier verandering in te brengen.\n\nDeze scriptie, diens titel vrij vertaalt naar \"Het Aanpassen van Microbiele Levenswijze\", is erop gericht om industrie\u00ebl toegepaste micro-organismen te verbeteren. Door zijn inherente robuustheid, grote redox capaciteit, aanpassingsvermogen en vrij verkrijgbaar genoom wordt de gram-negatieve prokaryote bacterie Pseudomonas putida gebruikt als model-organisme, maar het onderzoek is uitgebreidt met Escherichia coli en het eukaryote gist Saccharomyces cerevisiae.\n\nHoofdstuk 2 omschrijft het industri\u00eble landschap ten tijde van dit onderzoek. Dit onderzoek was erop gericht om bacteri\u00ebn te verbeteren vanuit een academisch oogpunt, maar voor industri\u00eble toepassing. Om te bepalen hoe ver deze velden uit elkaar liggen werd er een serie interviews gehouden met participanten zowel uit de academia als uit de industrie. Door hun compleet verschillende doelstellingen komen de meningen en prioriteiten van academia en industrie niet altijd overeen. De voornaamste punten van miscommunicatie en verschillende kansen zijn blootgelegd in dit hoofdstuk. Terwijl de industrie voornamelijk focust op de problemen van vandaag zoekt academia oplossingen voor de problemen van morgen. Beide partijen zijn het erover eens dat communicatie met rasse schreden verbeterd zou moeten worden, zodat meer uitvindingen vanuit het onderzoek toegepast worden.","summary":"We determine the effect of exchanging the L-aspartate oxidase gene in different mesophiles for a thermophilic ortholog. In E. coli, the exchange results in a 10 times shorter lag phase when grown at 44 \u00b0C. In P. putida the generation time doubled when grown at 40 \u00b0C. To determine how widespread the connection between NAD+ availability and thermo-tolerance is, the mesophilic or thermophilic aspartate pathway was implemented in yeast, which generates NAD+ via the kynurenine pathway. Not only was S. cerevisiae able to use the aspartate pathway for NAD+ generation, but supplemented with the bacterial pathway the strain proved more robust when grown at 41 \u00b0C.\n\nTo determine what effect this increased thermo-tolerance could have on an industrial scale, Chapter 7 describes the development of a millifluidics device. A down-scaled microfluidics system was built for this purpose. When kept at room temperature, the device can expose the cells to temperature fluctuations ranging from 25 \u00b0C to 50 \u00b0C. The system was applied to monitor long-term recombinant strain performance with respect to these fluctuations. The recombinant thermo-tolerant strains were tested in this system over a three-day span. It was seen that with a temperature fluctuation regime of 50-40-30-40 \u00b0C, P. putida KT244O \u2206nadB pS638 nadBBsm equipped with the thermophilic ortholog from B. smithii grows better than the other strains, including the wild type P. putida KT244O which is unburdened by a plasmid.\n\nThe discovery of the universal link between the NAD+ availability in microbes and their thermo-tolerance was patented. Appendix chapter 6 shows the patent. This invention relates to the field of molecular microbiology, metabolic engineering, and fermentation technology. In particular, this relates to microbial host cells that have been engineered for increased tolerance to temperature shifts, for increased performance at temperatures different from optimal temperature of a micro-organism and\/or for changing at least one of the cardinal temperatures of a micro-organism by replacing an endogenous NAD+ biosynthesis gene by a heterologous gene encoding a corresponding enzyme with another temperature profile. With the patent, a full trajectory from lab to test to application was completed.","auteur":"Linde Kampers","auteur_slug":"linde-kampers","publicatiedatum":"4 juni 2020","taal":"EN","url_flipbook":"https:\/\/ebook.proefschriftmaken.nl\/ebook\/lindekampers?iframe=true","url_download_pdf":"","url_epub":"","ordernummer":"FTP-202604060922","isbn":"978-94-6395-324-5","doi_nummer":"","naam_universiteit":"Wageningen University","afbeeldingen":13815,"naam_student:":"","binnenwerk":"","universiteit":"Wageningen University","cover":"","afwerking":"","cover_afwerking":"","design":""},"_links":{"self":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio\/8611","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio"}],"about":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/types\/us_portfolio"}],"author":[{"embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/comments?post=8611"}],"version-history":[{"count":1,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio\/8611\/revisions"}],"predecessor-version":[{"id":8614,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio\/8611\/revisions\/8614"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/media\/13815"}],"wp:attachment":[{"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/media?parent=8611"}],"wp:term":[{"taxonomy":"us_portfolio_category","embeddable":true,"href":"https:\/\/www.proefschriftmaken.nl\/en\/wp-json\/wp\/v2\/us_portfolio_category?post=8611"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}